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Lecture 02
DNA sequencing

Date: August 29, 2024

The session traces the evolution of DNA sequencing technologies, commencing with the seminal Sanger method and progressing to state-of-the-art Illumina and Nanopore platforms. Through a comparative analysis of these technologies, we will elucidate the rapid advancements that have catalyzed genomic research. The discourse will encompass a critical evaluation of the strengths and limitations inherent to each method, offering insights into their optimal applications in contemporary research contexts. The lecture culminates with an in-depth overview of a typical DNA sequencing workflow, providing students with a pragmatic understanding of genomic data generation.

Learning objectives

What you should be able to do after today's lecture:

  1. Analyze antibiotic resistance challenges and evaluate genomics' role in mitigating them.
  2. Construct a general workflow intrinsic to DNA sequencing experiments.
  3. Delineate the core principles underlying Sanger sequencing.
  4. Conduct a comparative analysis of Illumina sequencing vis-à-vis Sanger sequencing.
  5. Explicate the fundamental principles governing Nanopore sequencing technology.

Readings

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